Composite

Part:BBa_K912010:Design

Designed by: Grant Nicholas   Group: iGEM12_Northwestern   (2012-09-28)


BBa_J23104(strong) promoter/RBS+ Chloride antiporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 364
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
    Illegal AgeI site found at 1323
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No additional design considerations besides making primers


Source

CLC-EC1: PCR'd out from genome Constitutive promoter: parts registry RBS: parts registry

References